Which is the most suitable part of the plant used to make virus free plant in micropropagation?

meristem

Herein, which is the most suitable part of the plant used to make virus free plant in micro propagation?

axillary meristem

Similarly, how do you produce virus free plants? Shoot meristem-tip culture alone or in combination with thermotherapy, chemotherapy, electrotherapy and/or cryotherapy is being widely used to raise virus free plants from infected stock plants.

Additionally, which part would be most suitable for raising virus free plant?

meristem

Which meristem is used in plant tissue culture to obtain virus free plants?

Like other species, garlic shoot meristem culture has long been used for the production of virus-free plants (Walkey, 1990). However, the production and propagation rate of virus-free plantlets is very low (Ayabe and Sumi, 2001).

Related Question Answers

Which part would you select as an explant to recover healthy plants from virus affected plants?

if you want to regenerate through calli formation then you have wide choices to select from depending on plants. Mostly for dicots, leaf tissue is a good choice.

Which milk is used in tissue culture?

Coconut milk

What is micropropagation in biology?

Micropropagation refers to the in vitro multiplication and/or regeneration of plant material under aseptic and controlled environmental conditions to produce thousands or millions of plants for transfer to the field.

Why are meristems virus-free?

In the meristem there is no cells differentiation. Virus can't move from cell to cell through plasmodesmesta using their Movement protein.

How do you get rid of a plant virus?

Tissue culture of shoot tip meristems and heat therapy are used to eliminate viruses from infected plants. Due to the growth rate and cell structure of the apical meristems, viruses cannot fully function there and heat suppresses virus multiplication in most cases (viroids however are not always reduced by heat).

What is thermotherapy in plants?

Thermotherapy, simple in principle, consists in heat treatment of plant parts at temperature/time regimes that kill the conserved pathogen and that are only slightly injurious to the host. Heat is applied mainly by water, air, or vapor. To disinfect seeds, authors mainly use hot water and hot air treatments.

Why is cytokinin important in micropropagation?

Axillary bud multiplication is an effective alternative for clonal propagation. Cytokinin is one of the plant hormones crucial for plant growth and development and is known to promote cell division and differentiation[6].

What is root tip culture?

What is the Meaning of Root Culture? Root culture can be defined as the culture of excised radical tips of aseptically germinated seeds in a liquid medium where they are induced to grow independently under controlled conditions.

What is meant by Totipotency?

Totipotent. An isolated cell that is able to produce a fertile adult individual. An isolated cell is transferred to a uterus (after inserting into an empty zona pellucida or after developing to the blastocyst stage in culture) and it gives rise to a fertile adult.

Which gibberellin is essential for meristem culture?

Kinetin and benzyl-aminopurine are the most frequently used cytokinins in plant cell cultures. The Gibberellins is mainly used to induce plantlet formation from adventive embryos formed in culture.

How can haploid plants be produced?

Haploid plants can be produced through in vitro culture of male gametophytic cells at the microspore or immature pollen developmental stage. These cells respond in vitro by undergoing embryogenesis or haploid callus proliferation.

Who is the father of plant tissue culture?

Haberlandt

What is callus culture in plants?

Callus culture is the culture of dedifferentiated plant cells induced on media usually containing relatively high auxin concentrations or a combination of auxin and cytokinin under in vitro conditions.

What does apical meristem produce?

Apical meristem, region of cells capable of division and growth in the root and shoot tips in plants. Apical meristems give rise to the primary plant body and are responsible for the extension of the roots and shoots.

Which culture gives rise to haploid plants?

Anther culture